GEL ELECTROPHORESIS

INTRODUCTION

• Biologist use this chamber to identify different pieces of DNA.
• A macromolecule is a molecule that contains a large amount of atoms.
• The smaller the macromolecule the faster its speed.
• The bigger the macromolecule the slower it moves.
• This chamber determines the size and charge of the macromolecule.

HYPOTHESIS

REQUIREMENTS

NEEDS

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• Mix 200ml of bottled water & 2grams of baking soda.
• Set it aside because that's the Buffer Solution!

BAMNN

NEXT!

• Mix 100ml of Buffer Solution and 1gram of Agarose powder.
• Heat it up in the microwave and stir every 15 seconds.
• Take the gel out once it begins to bubble.
• Pour it into the rectangular container.
• Place the cardboard into the container and hold it there.

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GEL BEING

DRY FOR...

TAKE OUT

SEE THE 3 HOLES!?

PLACE METAL RODS ON BOTH ENDS.

GATHER 5 2-VOLTAGE BATTERIES!

HARD PART

• Strip 2 wires until the copper wire is visible.
• Strap each end of the wire to an alligator clip.
• One color per wire!

LIKE THIS!

THIS !

FINALE!

TADAAH !

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NOW THE MAGIC BEGINS....

CAN U SEE THE BUBBLES !?

45 MINUTES LATER...

RESULTS!

• Macromolecules have began to split into pieces
• Each food dye had a different speed because of its size.
• The green food dye had the greatest distance.
• There was 4 trials and the green dye won all of them!
• Bubbles appeared in so the chamber worked!

THAT IS HOW YOU DISTINGUISH DNA!

FIN