Disproved spontaneous generation of life by using two heated flasks full of broth. One flask closed, and the other open. The open one developed growth.
The Miller and Urey experiment was a chemical experiments thought to be simulating earths condition at that time. They discovered that some carbon turned into organic compound and proteins.
Oparin studied biochemestry if material processing done by plants, and enzyme reactions in plant cells. He showed that a lot of food production processes are based on bio catalysis.
Oparin thought that organic compounds could undergone reactions to make more complex molecules. He suggested molecules forked colloid aggregates. These were able to absorb and assimilate organic compounds from the environment reminiscent to metabolism. These would of evolved and became first life forms.
In the beginning of science about life's origin, they though living could come from non living. Pasteur disproved that with his experiments. Then Miller and Urey tried simulating earths conditions, making some proteins and adenine. Later, Oparin found ways of which simple cells became more complex.