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Project idea

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PRESENTATION OUTLINE

PROJECT IDEA.

JAMILA, KHLOUD, MNEEERA, NOURA OBAID.
Photo by IRRI Images

Our idea is: determining the effect of pomegranate on treating diarrhea that's caused by E.coli.

Photo by jar [o]

BACK GROUND

  • (NaturalNews) A small deciduous tree or large shrub that bears fruit, the Punica granatum, commonly known as the pomegranate, is a superfood with a long and rich history. Native to the East, it can be traced through historical documents as far back as 4000 B.C. The red fruit grows from pretty red flowers and is between a lemon and a grapefruit in size. The white flesh inside the thick skin is full of several hundred seeds.
Photo by CIMMYT

BACK GROUND

  • Pomegranates are grown as both a fruit crop and as ornamental trees or shrubs for parks and gardens. They are tolerant of drought and moderate frost, growing in climates similar to the Mediterranean region, California, or Northern India. There are many genotypes, with the differences ranging from exocarp and aril color, hardness of seed, fruit size, juice content and astringency, and so on. The pomegranate has become increasingly popular in the countries of orth America over the last few decades
Photo by CIMMYT

MAKING THE LB AGAR PLATE

  • 1. Add 250 mL of dH2O to a graduated cyclindar. 2. Weigh out 20g of premix LB Agar powder (VWR DF0445-17) or: 3. 5.0 g tryptone 4. 2.5 g yeast extract 5. 5.0 g NaCl 6. 7.5 g agar 7. Mix powder well to bring into solution

MAKING THE LB AGAR PLATE

  • 8. Add dH2O to total volume of 500 mL and transfer to 1 L flask 9. Put on stirring hot plate and heat to boil for 1 min while stirring. 10. Transfer to 1 L pyrex jar and label with autoclave tape. 11. Autoclave at liquid setting for 20 minutes in a basin making sure to loosen top 12. Let agar cool to ~55C (you should be able to pick up the jar without a glove)

How to count the bacteria

  • primary trick in colony-counting is to count each colony dot once. One approach is to set the Petri dish on a gridded background and count the cells in each grid cell, moving in a methodical pattern through all of the cells. Marking counted colonies on the back of the Petri dish can also be a helpful approach. Generally, you will need to count at least three plates; only use plates containing 30 to 300 colonies to make robust inferences.

Materials and equipments

  • E-coli bacteria
  • Petri dishes
  • 3 Agar plates
  • Pestle and mortar
  • Gloves
  • Pomegranate powder
  • Imodium tablets
  • Microscopet
  • Incubator. 10. Pipet
Photo by eltpics

METHOD :

  • 1.We will bring three Petri dish which contain (E.coil) bacteria. 2.In the first Petri dish we will apply pomegranate , in the second one we will add Imodium and thirds one is the control bacteria.
Photo by Josh Kenzer

METHOD :

  • 3.We will add certain dose of pomegranate and Imodium every day. We will compare the results between pomegranate and Imodium and we will decide which is more effective.
  • 4.We will design to collect the data so we Codifies the observational results.
Photo by Josh Kenzer

METHOD:

Photo by Wiedmaier

DIAGRAM OF APPARATUS

Photo by Wiedmaier

Health and safety precautions

  • 1.work in a well-ventilated area. 2.A lab coat or smock should be worn during. 3.laboratory experiments. 4.Eye protection or goggles are worn whenever risk assessments require it. 5.Hand-washing and eye wash facilities are readily available in the laboratory. 6.Gloves should be worn before beginning the experiment.

Applications link to our culture

  • Pomegranates and the juice from the arils have been hailed for years as a sure way to cure diarrhea. Ancient writings refer to the use of this truly amazing fruit as a natural way to stop diarrhea, help with stomach ailments in general, and stopping blood from passing with stool in diarrhea. Pomegranate juice functions as a natural astringent inside the body, which means it could be quite effective for treating diarrhea in place of an over-the-counter medication.
Photo by nathanmac87