Hershey And Chase by Grace Reynolds

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Slide Notes

Hershey And Chase

Published on Nov 20, 2015

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PRESENTATION OUTLINE

HERSHEY AND CHASE

ASHLEY WILLIAMS AND GRACE REYNOLDS

Photo by kevin dooley

MARTHA CHASE AND ALFRED HERSHEY

Hershey and Chase started a experient on T2 phage, a virus that has a structure of a protein shell that contains genetic material.

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The phage infects a bacterium by attaching to its outer membrane and injecting its genetic material and leaving its empty shell attached to the bacterium.

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radioactive Phosphorus-32

FIRST EXPERIMENT

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They allowed the phages to infect E. coli, and through several elegant experiments were able to observe the transfer of P32 labeled phage DNA into the cytoplasm of the bacterium.

Sulfur-35

SECOND EXPERIMENT

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Following infection of E. coli they then sheared the viral protein shells off of infected cells using a high-speed blender and separated the cells and viral coats by using a centrifuge. After separation, the radioactive S35 tracer was observed in the protein shells, but not in the infected bacteria, supporting the hypothesis that the genetic material which infects the bacteria was DNA and not protein.

SOURCES :

http://www.princeton.edu/~achaney/tmve/wiki100k/docs/Hershey-Chase_experime...

Photo by Will Montague

Grace Reynolds