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DNA Replication

Published on Nov 22, 2015

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PRESENTATION OUTLINE

DNA REPLICATION

MIRANDA HILD

STEP BY STEP REPLICATION

1. The DNA double helix needs to unwind and the two strands need to seperate.
2. The enzyme helicase is responsible for this process.
3. Free nucleotides which are floating around in the nucleus form complementary pairs with the nucleotides of both of the DNA strands.

4. The nucleotides which have formed complementary base pairs with the nucleotides on the old strand now join to each other to form a new strand or DNA. (DNA polymerase is responsible for this)
5. The ligase attaches to the Okazaki DNA to form one strand.
6. This all results in two identical double helix strands. Each have one old strand and one new strand.

LEADING STRAND

The leading strand is the strand at the opposite side of the replication fork from the lagging strand. The leading strand runs from 5 to 3 ends.

LAGGING STRAND

The lagging strand is called the lagging strand because it lags behind the leading strand.
In replication, RNA primer applies the primer to the lagging strand. Once the primer is down DNA polymerase adds nucleotides to the lagging strand. Next, DNA ligase attaches the Okazaki fragments together.

OKAZAKI FRAGMENTS

The lagging strand is the strand that breaks into pieces called Okazaki fragments. For the Okazaki fragments to form, RNA primase lays down an RNA primer. Then polymerase lays down new DNA in the form of nucleotides. Polymerase replaces the RNA primers with DNA. Lastly, DNA ligase connects the Okazaki fragments together.

SEMI-CONSERVATIVE REPLICATION

DNA replication is a semi-conservative replication because it produces two copies that each have one old strand and one new strand.

DNA REPLICATIONS IMPORTANCE

DNA replication is important because it is needed when a cell divides. The replication allows the cell to be duplicated so that it can continue the process of the cell.