Crossmatching by Judit Homolya

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Slide Notes

Crossmatching

Published on Mar 29, 2016

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PRESENTATION OUTLINE

CROSSMATCING

To detect previously sensitized recipients and those with natural antibodies
To prevent administration of incompatible blood
99 % cats - blood group A
Abyssinian, Birman, British Shorthair, Devon Rex, Himalayan, Persian, Scottish Fold, and Somali, have a higher frequency of blood group B
incompatible transfusion in cats results in rapid destruction of transfused cells

MIC ANTIGEN

present in some cats, and naturally occurring antibodies are present in cats that lack the mic antigen
crossmatching should be performed for cats before the first transfusion
In dogs Crossmatching is only used with subsequent transfusion, no naturally occurring antibodies

MAJOR CROSSMATCH

detects antibodies already present in recipient plasma
Anticoagulant is added to blood samples from donor and recipient
donor RBCs are washed 3 times with 0.9% saline, and a 4% RBC suspension in saline is made from the washed cells
combining equal volumes (0.1 mL) of the donor RBC suspension and recipient plasma
samples are incubated, centrifuged, and evaluated for hemolysis or agglutination

EVALUATION

evaluated by comparing the color of the supernatant with control sample
then gently shaken until all cells in have returned to suspension
the degree of cell clumping is compared with the control sample
negative, or compatible - plasma is clear and the RBCs are readily suspended
positive, or incompatible - hemolysis or hemagglutination or both

MINOR CROSSMATCHING

reverse of the major crossmatch, recipient cells are combined with donor plasma
important only in species such as cats with clinically significant naturally occurring isoantibodies
or if the donor has been previously transfused
horses, those with previous pregnancies

Untitled Slide

agglutination technique is adequate for the dog and cat
horse has both agglutinating and hemolytic antibodies, procedures testing for both are warranted
hemolytic test is necessary for cattle, sheep, and goats since they have minimal agglutinating antibodies

SAMPLE SUBMISSION

Recipient samples for crossmatch or blood typing tests should be submitted in EDTA tubes
Donor samples can be submitted in EDTA or as blood bag segments

Judit Homolya

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